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A Label-free and Highly Sensitive Fluorescence Strategy for Mercury Ion Detection Based on Exonuclease III-aided Recycling Amplification.

Anal Sci. 2018-01; 
DingBaomiao, LiuChenguang, WuQinghua, WangYun, LiLi, YangHu
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Custom DNA/RNA Oligos … Also, this method can be devised utilizing both the advantages of label-free architectures and signal-on architectures. Experimental. Materials and chemicals. Oligonucleotides (guaranteed oligos, HPLC-purified) were purchased from Genscript Co., Ltd. (Nanjing, China) … Get A Quote

摘要

In this paper, we describe a simple and highly sensitive fluorescence strategy of mercury ions based on exonuclease III (Exo III)-aided target recycling amplification to ensure sensitivity. With an ultra high sensitivity (1 pM), our strategy has been simple and cost-effective, which does not need any artificial modification fluorescence groups, and can be carried out in a pot. It also shows excellent selectivity. Therefore, our new method provides an effective platform for mercury-ion detection.

关键词

G-quadruplex DNA,Mercury ion detection,T-Hg-T mismatches,exonuclease