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| Proteins, Expression, Isolation and Analysis | … The lysate was precleared with Protein G magnetic beads (GenScript, Piscataway, NJ, USA) at 4 °C for 1 h. A total of 4 μg of rabbit anti-NFE2L1 (sc721) or anti- SRP54 (AB154796) was added and incubated at 4 °C over- night with gentle rotation … | Get A Quote |
A promoter can be regulated by various cis-acting elements so that delineation of the regulatory modes among them may help understand developmental， environmental and genetic mechanisms in gene activity. Here we report that the human dopamine transporter gene SLC6A3 carries a 5' distal 5-kb super enhancer (5KSE) which upregulated the promoter by 5-fold. Interestingly， 5KSE is able to prevent 3' downstream variable number tandem repeats (3'VNTRs) from silencing the promoter. This new enhancer consists of a 5'VNTR and three repetitive sub-elements that are conserved in primates. Two of 5KSE's sub-elements， E-9.7 and E-8.7， upregulate the promoter， but only the later could continue doing so in the presen... More
