Bacillithiol is a low molecular weight thiol found in Firmicutes that is analogous to glutathione， which is absent in these bacteria. Bacillithiol transferases catalyze the transfer of bacillithiol to various substrates. The S-transferase-like (STL) superfamily contains over 30，000 putative members， including bacillithiol transferases. Proteins in this family are extremely divergent and are related by structural rather than sequence similarity， leaving it unclear if all share the same biochemical activity. Bacillus subtilis encodes eight predicted STL superfamily members， only one of which has been shown to be a bacillithiol transferase. Here we find that the seven remaining proteins show varying leve... More
Bacillithiol is a low molecular weight thiol found in Firmicutes that is analogous to glutathione， which is absent in these bacteria. Bacillithiol transferases catalyze the transfer of bacillithiol to various substrates. The S-transferase-like (STL) superfamily contains over 30，000 putative members， including bacillithiol transferases. Proteins in this family are extremely divergent and are related by structural rather than sequence similarity， leaving it unclear if all share the same biochemical activity. Bacillus subtilis encodes eight predicted STL superfamily members， only one of which has been shown to be a bacillithiol transferase. Here we find that the seven remaining proteins show varying levels of metal dependent bacillithiol transferase activity. We have renamed the eight enzymes BstA-H. Mass spectrometry and gene expression studies revealed that all of the enzymes are produced to varying levels during growth and sporulation， with BstB and BstE being the most abundant and BstF and BstH being the least abundant. Interestingly， several bacillithiol transferases are induced in the mother cell during sporulation. A strain lacking all eight bacillithiol transferases showed normal growth in the presence of stressors that adversely affect growth of bacillithiol-deficient strains， such as paraquat and CdCl2. Thus， the STL bacillithiol transferases represent a new group of proteins that play currently unknown， but potentially significant roles in bacillithiol-dependent reactions. We conclude that these enzymes are highly divergent， perhaps to cope with an equally diverse array of endogenous or exogenous toxic metabolites and oxidants.
