Activation of Macrophages in vitro by Phospholipids from Brain of Katsuwonus pelamis (Skipjack Tuna).

The biological activities of phospholipids (PLs) have attracted people's attention， especially marine phospholipids with omega-3 polyunsaturated fatty acids DHA and EPA. In this study， we investigated the immunity activation of macrophages in vitro by phospholipids from skipjack brain. The phospholipids were extracted with hexane and ethanol ultrasonication instead of the traditional method of methanol and chloroform. The content of phospholipids from Skipjack brain was 19.59 g/kg by the method (the ratio of hexane and ethanol 2:1， 40 min， 35°C， 1:9 of the ratio of material to solvent， ultrasonic power 300W， ultrasonic extraction 2 times). The RAW264.7 macrophages were stimulated by the phospholipids from the Skipjack， by which the volume， viability and phagocytosis of macrophages were increased. The concentration of NO and the activity of SOD of the cells were also enhanced. The gene expressions of IL-1β， IL-6， iNOS and TNF-α mRNA assayed by RT-PCR were up-regulated. Phospholipids from brain of Skipjack Tuna could activate macrophages immunity which displayed to induce pro-inflammatroy cytokines mRNA expression.