| Products/Services Used | Details | Operation |
|---|---|---|
| Proteins, Expression, Isolation and Analysis | … used were as follows: Dmrt1 (1:500; Santa Cruz, CA); Plzf (1:200; eBioscience, Inc., San Diego, CA); c-kit (1:200; Boster, Wuhan, China); Stra8 (1:200; Abcam, Cambridge, MA); Plzf (1:300; Sino Biological, Inc., Shanghai, China); Dazl (1:100; Genscript, Nanjing, China); Rarg (1 … | Get A Quote |
The protein encoded by double sex and mab-3 related transcription factor 1 (Dmrt1) gene contains a double sex/mab-3 domain， which was considered as one of the most conservative structures in sex determination. However， its effect on spermatogenesis of dairy goat spermatogonial stem cells (SSCs) remains to be clarified. For the first time， the roles of Dmrt1 in spermatogenesis of livestock are highlighted. Here， we investigated the expression pattern of Dmrt1 in the testes of dairy goats. Dmrt1 primarily located in undifferentiated SSCs. Moreover， Dmrt1 enhanced differentiation and proliferation of mGSCs. On the contrary， the level of meiosis was down-regulated， as Dmrt1 determines whether SSCs und... More
