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Peptide Synthesis> | ... TIR domains of TLR1 and TLR6 are designated as 16R1, 16R4, 16R6, and 16R7. Peptides of more than 95% purity were synthesized by AAPPTec (Louisville, KY) or GenScript (Piscataway, NJ), and were quantified by spectrophotometry (Pace et al. 1995). Expression vectors ... | Get A Quote |
TLR2 heterodimers with TLR1 or TLR6 recognize distinct pathogen-associated molecules such as tri- and di-acylated lipopeptides. The activated TLR2 heterodimers recruit Toll-IL-1R domain- (TIR-) containing adapter proteins, TIRAP and MyD88, through the receptor TIR domains. Molecular recognition mechanisms responsible for agonist-driven, TIR domain-mediated receptor-adapter interactions as well as the structure of resultant signaling complexes remain unknown. We previously reported that the cell-permeable peptide derived from helix D of TLR2 TIR (2R9) specifically binds TIRAP in vitro and in cells and thereby inhibits TIRAP-dependent TLR signaling. This study demonstrates that cell-permeable peptides from D heli... More