| Products/Services Used | Details | Operation |
|---|---|---|
| Peptide Synthesis | ... residue is underlined). After booster immunizations, mice were sacrificed and splenocytes isolated from the spleen and fused with myeloma cells following the hybridoma generation methodology (Genscript). Clones recognizing exclusively the phosphorylated peptide over the ... | Get A Quote |
Coronins constitute a family of conserved proteins expressed in all eukaryotes that have been implicated in the regulation of a wide variety of cellular activities. Recent work showed an essential role for coronin 1 in the modulation of the cAMP/PKA pathway in neurons through the interaction of coronin 1 with the G protein subtype Gαs in a stimulus-dependent manner, but the molecular mechanism regulating coronin 1-Gαs interaction remains unclear. We here show that phosphorylation of coronin 1 on Thr(418/424) by cyclin-dependent kinase (CDK) 5 activity was responsible for coronin 1-Gαs association and the modulation of cAMP production. Together these results show an essential role for CDK5 activity in promoti... More
