RNA silencing plays important roles in the regulation of gene expression in eukaryotes. We previously reported that RNA silencing of a linked endogenous gene and a transgene in transgenic rice seeds can be induced by the expression of foreign recombinant mGLP-1, which acts as a silencing-inducible sequence through RNA interference. In this study, we found that the induction of RNA silencing by foreign transgenes is not restricted to mGLP-1 but is observed in many other genes as a relatively general phenomenon, as several foreign genes were involved in inducing RNA silencing in the same manner as mGLP-1 in transgenic rice. We detected 21-24-nt siRNAs using both sense and antisense probes specific to the silenced... More
RNA silencing plays important roles in the regulation of gene expression in eukaryotes. We previously reported that RNA silencing of a linked endogenous gene and a transgene in transgenic rice seeds can be induced by the expression of foreign recombinant mGLP-1, which acts as a silencing-inducible sequence through RNA interference. In this study, we found that the induction of RNA silencing by foreign transgenes is not restricted to mGLP-1 but is observed in many other genes as a relatively general phenomenon, as several foreign genes were involved in inducing RNA silencing in the same manner as mGLP-1 in transgenic rice. We detected 21-24-nt siRNAs using both sense and antisense probes specific to the silenced genes in both the leaves and endosperm of transgenic rice plants. Moreover, read-through transcripts were consistently observed in silenced transgenic rice plants. Taken together, these results suggest that proper transcription termination was prevented in these plants, and the highly divergent 3′-end transcripts served as templates for double-stranded RNA synthesis, resulting in the degradation of the target genes via siRNA.
