| Species |
Cynomolgus&Mouse |
| Protein Construction |
| His |
IL-23 alpha & IL-12 beta [Val22-Pro189 (Cynomolgus IL-23 alpha) & Met23-Ser335 (Mouse IL-12 beta)]
Accession # A0A2K5TLV4-1 (Cynomolgus IL-23 alpha) & P43432-1 (Mouse IL-12 beta) |
| N-term |
C-term |
|
| Purity |
> 95% as determined by BisTris PAGE
> 95% as determined by HPLC |
| Endotoxin Level |
Less than 1EU per μg by the LAL method. |
| Biological Activity |
Measured by its binding ability in a functional ELISA. Immobilized IL-23 alpha&IL-12 beta, His, Cynomolgus&Mouse at 2μg/ml (100μl/well) on the plate can bind Human IL23R, hFc Tag. Test result was comparable to standard batch. |
| Expression System |
HEK293 |
| Theoretical Molecular Weight |
19.5 (Cynomolgus IL-23 alpha)&35.8 (Mouse IL-12 beta) kDa |
| Apparent Molecular Weight |
Due to glycosylation, the protein migrates to 22 (Cynomolgus IL23 alpha)&40-50 (Mouse IL12 beta) kDa based on Bis-Tris PAGE result. |
| Formulation |
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). |
| Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Storage & Stability |
Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles. |
| Target Background |
Interleukin (IL)-12 and IL-23 belong to the IL-12 type family and are composite cytokines, consisting of the common β subunit p40 and the specific cytokine α subunit p35 and p19, respectively. IL-12 signals via the IL-12Rβ1·IL-12Rβ2 receptor complex, and IL-23 uses also IL-12Rβ1 but engages IL-23R as second receptor. |
| Synonyms |
IL23 alpha; IL12 beta; IL23 alpha&IL12 beta ; IL12 β; IL23 alpha&IL12 β |
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
