IL-23 alpha&IL-12 beta, His, Cynomolgus&Mouse

Interleukin (IL)-12 and IL-23 belong to the IL-12 type family and are composite cytokines, consisting of the common β subunit p40 and the specific cytokine α subunit p35 and p19, respectively. IL-12 signals via the IL-12Rβ1·IL-12Rβ2 receptor complex, and IL-23 uses also IL-12Rβ1 but engages IL-23R as second receptor.

价格	￥5200 在线订购包邮(港澳台除外)
产品编号	Z04837-100
规格	100 μg 500 μg 大单询价
数量
参考货期


咨询产品问题

产品简介图例技术背景

产品简介

Species

Cynomolgus&Mouse

Protein Construction

His	IL-23 alpha & IL-12 beta [Val22-Pro189 (Cynomolgus IL-23 alpha) & Met23-Ser335 (Mouse IL-12 beta)] Accession # A0A2K5TLV4-1 (Cynomolgus IL-23 alpha) & P43432-1 (Mouse IL-12 beta)
N-term	C-term

Purity

> 95% as determined by BisTris PAGE

Endotoxin Level

Less than 1EU per μg by the LAL method.

Biological Activity

Measured by its binding ability in a functional ELISA.Test result was comparable to standard batch.

Expression System

HEK293

Theoretical Molecular Weight

19.5 (Cynomolgus IL-23 alpha)&35.8 (Mouse IL-12 beta) kDa

Apparent Molecular Weight

Due to glycosylation, the protein migrates to 22 (Cynomolgus IL23 alpha)&40-50 (Mouse IL12 beta) kDa based on Bis-Tris PAGE result.

Formulation

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4).

Reconstitution

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Storage & Stability

Upon receiving, the product remains stable for 6 months at -20℃ or below. Upon reconstitution, the product should be stable for 3 months at -80℃. Avoid repeated freeze-thaw cycles.

图例

Immobilized IL-23 alpha&IL-12 beta, His, Cynomolgus&Mouse, His Tag at 2 μg/ml (100 μl/well) on the plate. Dose response curve for Human IL-23R, hFc Tag with the EC50 of 27.5ng/ml determined by ELISA. »

The purity of IL-23 alpha&IL-12 beta, His, Cynomolgus&Mouse is greater than 95% as determined by SEC-HPLC. »

IL-23 alpha&IL-12 beta, His, Cynomolgus&Mouse on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. »

IL-23 alpha&IL-12 beta, His, Cynomolgus&Mouse, His Tag immobilized on CM5 Chip can bind Cynomolgus IL-23 alpha&Mouse IL-12 beta, His Tag with an affinity constant of 3.90 nM as determined in SPR assay (Biacore T200). »

技术背景

Target Background	Interleukin (IL)-12 and IL-23 belong to the IL-12 type family and are composite cytokines, consisting of the common β subunit p40 and the specific cytokine α subunit p35 and p19, respectively. IL-12 signals via the IL-12Rβ1·IL-12Rβ2 receptor complex, and IL-23 uses also IL-12Rβ1 but engages IL-23R as second receptor.
Synonyms	IL23 alpha; IL12 beta; IL23 alpha&IL12 beta ; IL12 β; IL23 alpha&IL12 β

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.