| ￥5200 | |
| Z04837-100 | |
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| Species | Cynomolgus&Mouse | ||||
| Protein Construction |
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| Purity | > 95% as determined by BisTris PAGE | ||||
| Endotoxin Level | Less than 1EU per μg by the LAL method. | ||||
| Biological Activity | Measured by its binding ability in a functional ELISA.Test result was comparable to standard batch. | ||||
| Expression System | HEK293 | ||||
| Theoretical Molecular Weight | 19.5 (Cynomolgus IL-23 alpha)&35.8 (Mouse IL-12 beta) kDa | ||||
| Apparent Molecular Weight | Due to glycosylation, the protein migrates to 22 (Cynomolgus IL23 alpha)&40-50 (Mouse IL12 beta) kDa based on Bis-Tris PAGE result. | ||||
| Formulation | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). | ||||
| Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. | ||||
| Storage & Stability | Upon receiving, the product remains stable for 6 months at -20℃ or below. Upon reconstitution, the product should be stable for 3 months at -80℃. Avoid repeated freeze-thaw cycles. |
Immobilized IL-23 alpha&IL-12 beta, His, Cynomolgus&Mouse, His Tag at 2 μg/ml (100 μl/well) on the plate. Dose response curve for Human IL-23R, hFc Tag with the EC50 of 27.5ng/ml determined by ELISA. »
The purity of IL-23 alpha&IL-12 beta, His, Cynomolgus&Mouse is greater than 95% as determined by SEC-HPLC. »
IL-23 alpha&IL-12 beta, His, Cynomolgus&Mouse on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. »
IL-23 alpha&IL-12 beta, His, Cynomolgus&Mouse, His Tag immobilized on CM5 Chip can bind Cynomolgus IL-23 alpha&Mouse IL-12 beta, His Tag with an affinity constant of 3.90 nM as determined in SPR assay (Biacore T200). »
| Target Background | Interleukin (IL)-12 and IL-23 belong to the IL-12 type family and are composite cytokines, consisting of the common β subunit p40 and the specific cytokine α subunit p35 and p19, respectively. IL-12 signals via the IL-12Rβ1·IL-12Rβ2 receptor complex, and IL-23 uses also IL-12Rβ1 but engages IL-23R as second receptor. |
| Synonyms | IL23 alpha; IL12 beta; IL23 alpha&IL12 beta ; IL12 β; IL23 alpha&IL12 β |
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
