IL-22[Biotin], His & Avi, Mouse

The cytokine interleukin-22 (IL-22) is a critical regulator of epithelial homeostasis. It has been implicated in multiple aspects of epithelial barrier function, including regulation of epithelial cell growth and permeability, production of mucus and antimicrobial proteins (AMPs), and complement production.

价格	￥5800 在线订购包邮(港澳台除外)
产品编号	Z04670-100
规格	100 μg 500 μg 大单询价
数量
参考货期


咨询产品问题

产品简介图例技术背景

产品简介

Species

Mouse

Protein Construction

Avi	His	IL-22 (Leu34-Val179) Accession # Q9JJY9
N-term		C-term

Conjugate

Biotin

Purity

> 95% as determined by BisTris PAGE

Endotoxin Level

Less than 1EU per μg by the LAL method.

Biological Activity

Measured by its binding ability in a functional ELISA.Test result was comparable to standard batch.

Expression System

E.coli

Theoretical Molecular Weight

19.7 kDa

Apparent Molecular Weight

The protein has a predicted MW of 19.7 kDa same as Bis-Tris PAGE result.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4).

Reconstitution

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Storage & Stability

Upon receiving, the product remains stable for 6 months at -20℃ or below. Upon reconstitution, the product should be stable for 3 months at -80℃. Avoid repeated freeze-thaw cycles.

图例

Loaded Mouse IL-22R alpha 1, hFc Tag on ProA-Biosensor can bindIL-22[Biotin], His & Avi, Mouse, His-Avi Tag with an affinity constant of 13.50 nM as determined in BLI assay (Gator® Prime). »

Immobilized IL-22[Biotin], His & Avi, Mouse, His Tag at 2 μg/ml (100 μl/well) on the streptavidin precoated plate(5 μg/ml). Dose response curve for Mouse IL-22R alpha 1, hFc Tag with the EC50 of 14.8ng/ml determined by ELISA. »

The purity of IL-22[Biotin], His & Avi, Mouse is greater than 90% as determined by SEC-HPLC. »

IL-22[Biotin], His & Avi, Mouse on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. »

IL-22[Biotin], His & Avi, Mouse, His Tag immobilized on CM5 Chip can bind Biotinylated Mouse IL-22, His Tag with an affinity constant of 0.145 μM as determined in SPR assay (Biacore T200). »

技术背景

Target Background	The cytokine interleukin-22 (IL-22) is a critical regulator of epithelial homeostasis. It has been implicated in multiple aspects of epithelial barrier function, including regulation of epithelial cell growth and permeability, production of mucus and antimicrobial proteins (AMPs), and complement production.
Synonyms	Interleukin-22; IL-22; Cytokine Zcyto18; IL-TIF; IL-D110; TIFa; TIFIL-23; ZCYTO18

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.