Species | Human | ||||||
Protein Construction |
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Conjugate | Biotin | ||||||
Purity |
> 95% as determined by BisTris PAGE > 95% as determined by HPLC |
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Endotoxin Level | Less than 1EU per ug by the LAL method. | ||||||
Biological Activity | Measured by its binding ability in a functional ELISA. Immobilized Human IL5, His Tag at 2μg/ml (100μl/well) on the plate can bind IL-5 R alpha/CD125[Biotin], His & Avi, Human. Test result was comparable to standard batch. | ||||||
Expression System | HEK293 | ||||||
Theoretical Molecular Weight | 38.7 kDa | ||||||
Apparent Molecular Weight | Due to glycosylation, the protein migrates to 55-60 kDa based on Bis-Tris PAGE result. | ||||||
Formulation | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). | ||||||
Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. | ||||||
Storage & Stability | Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles. |
Immobilized Human IL-5, His Tag at 2 μg/ml (100 μl/well) on the plate. Dose response curve for IL-5 R alpha/CD125[Biotin], His & Avi, Human, His Tag with the EC50 of 0.15 μg/ml determined by ELISA. »
The purity of IL-5 R alpha/CD125[Biotin], His & Avi, Human is greater than 95% as determined by SEC-HPLC. »
IL-5 R alpha/CD125[Biotin], His & Avi, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. »
Target Background | Interleukin‑5 Receptor alpha (IL‑5 R alpha), also known as CD125, is a 60 kDa hematopoietin receptor that plays a dominant role in eosinophil biology.This is the receptor for interleukin-5. The alpha chain binds to IL5. |
Synonyms | CD125; CDw125CD125; HSIL5R3; IL-5 R alpha; IL-5R subunit alpha; IL5R; IL5RA; IL-5Ra; MGC26560 |
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.