目录产品 » 2B4/CD244/SLAMF4[Biotin], His & Avi, Human
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2B4/CD244/SLAMF4[Biotin], His & Avi, Human

2B4 (CD244) is expressed by memory-phenotype CD8(+) T cells and all natural killer (NK) cells. The ligand for 2B4, CD48, is expressed on hematopoietic cells. 2B4 is conserved in humans and mice, and a number of reports have linked 2B4 with activation of lymphocytes.Engagement of 2B4 on NK cell surfaces with specific antibodies or CD48 can trigger cell mediated cytotoxicity, interferon γ secretion, phosphoinositol turnover and NK cell invasiveness.
￥5800
Z04382-100

产品简介
Species Human
Protein Construction
2B4/CD244/SLAMF4 (Cys22-Arg221)
Accession # Q9BZW8-2		 His Avi
N-term C-term
Conjugate Biotin
Purity > 95% as determined by Bis­Tris PAGE 
> 95% as determined by HPLC
Endotoxin Level Less than 1EU per μg by the LAL method.
Biological Activity Measured by its binding ability in a functional ELISA. Immobilized 2B4/CD244/SLAMF4[Biotin], His & Avi, Human at 0.5μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml) can bind Human CD48, hFc Tag. Test result was comparable to standard batch.
Expression System HEK293
Theoretical Molecular Weight 24.9 kDa
Apparent Molecular Weight Due to glycosylation, the protein migrates to 48-70 kDa based on Bis-Tris PAGE result.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4).
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage & Stability Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles.
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图例
2B4/CD244/SLAMF4[Biotin], His & Avi, Human

Immobilized 2B4/CD244/SLAMF4[Biotin], His & Avi, Human, His Tag at 1 μg/ml (100 μl/Well) on streptavidin(5 μg/ml) precoated plate. Dose response curve for Human CD48, hFc Tag with the EC50 of 2.9 ng/ml determined by ELISA. »

2B4/CD244/SLAMF4[Biotin], His & Avi, Human

The purity of 2B4/CD244/SLAMF4[Biotin], His & Avi, Human is greater than 95% as determined by SEC-HPLC. »

2B4/CD244/SLAMF4[Biotin], His & Avi, Human

2B4/CD244/SLAMF4[Biotin], His & Avi, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. »

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技术背景
Target Background 2B4 (CD244) is expressed by memory-phenotype CD8(+) T cells and all natural killer (NK) cells. The ligand for 2B4, CD48, is expressed on hematopoietic cells. 2B4 is conserved in humans and mice, and a number of reports have linked 2B4 with activation of lymphocytes.Engagement of 2B4 on NK cell surfaces with specific antibodies or CD48 can trigger cell mediated cytotoxicity, interferon γ secretion, phosphoinositol turnover and NK cell invasiveness.
Synonyms 2B4; CD244 antigen; CD244; h2B4; NAIL; NKR2B4; Nmrk; SLAMF4
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For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.