GenLNP-CX175-CD7VHH-CD19 CAR circRNA

GenLNP-CX175-CD7VHH-CD19 CAR circRNA is a CD7-targeted LNP formulated with CX175 and loaded with anti-human CD19 CAR circular RNA. Featuring CD7 VHH-mediated targeting, it enables efficient delivery to CD7+ immune cells for CAR expression and targeted circRNA delivery research.

价格	￥8000 在线订购包邮(港澳台除外)
产品编号	RP-A00084-50
规格	0.05 mg 大单询价
数量
参考货期


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产品属性质控参数应用指导图例

Description

The GenLNP-CX175-CD7VHH-CD19 CAR circRNA is a CD7 VHH-decorated lipid nanoparticle (LNP) loaded with circular RNA encoding an anti-human CD19 chimeric antigen receptor (CAR). The LNP displays anti-human CD7 VHH antibody (CD7-VHH-hIgG1Fc (LALA)) on its surface to enhance targeting efficiency toward CD7-positive cells. CD7 is highly expressed on the majority of mature T cells, NK cells, and their early precursors, making it an attractive target for selective delivery both in vitro and in vivo. The antibody is covalently conjugated to lipid molecules to achieve targeted delivery.

This LNP features:
- Formulation with CX175, a licensable LNP from GenScript, as the main ionizable lipid for efficient encapsulation and delivery
- CD7 VHH-mediated targeting for enhanced delivery to CD7+ immune cells
- Scarless anti-human CD19 CAR circular RNA (CVB3) payload designed without linker or junction sequences
- Optimized formulation for targeted circRNA delivery and immune cell engineering applications

This product enables efficient delivery of CD19 CAR circRNA to CD7+ cells and is ideal for CAR expression studies, immune cell engineering research, and targeted circRNA delivery applications.

产品属性

Form	Liquid
Concentration	0.15mg/mL
Full mRNA Molecular Weight	730168 Da
Full circRNA length	2265 nt
Storage buffer	Tris-HCl/Sucrose
Storage condition	Store at -80°C for long term. Avoid freeze thaw, only thaw once before use.

质控参数

Appearance	Clear and free of foreign particles
Encapsulation Efficiency	> 85%
Endotoxin	< 4 EU/ml
Particle Size	65-165 nm
pH	7.4 ± 0.5
Polydispersity Index	< 0.2
Zeta Potential	±20 mV

应用指导

1. LNP can be added directly to cells incubated in complete media
2. Cells grown and transfected in complete media (DMEM +10% FBS) has higher expression than reduced serum medium
3. For suspension cell lines, we recommend using low fluorescent background media such as Flourobrite® with 10% FBS, 1X GlutaMax® and 1X sodium pyruvate
4. For adherent cell line, we recommend replace complete media with Opti-mem right before reading the cell eGFP expression
5. For higher expression, we recommend incubate the LNP with FBS at 1:2 volume ratio (LNP:FBS) for 30 minutes at 37C before transfecting

图例

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.