scarless CD19 CAR circular RNA (CVB3)

Scarless CD19 CAR circRNA (CVB3) encodes a CAR with no linker or junction sequences. Enables transient, non-integrating CAR expression for immune cell engineering with a clean, minimal RNA design.

价格	￥2000
产品编号	RP-A00081-0.05
规格	0.05 mg 大单询价
数量

产品属性质控参数应用指导图例

Description

The scarless CD19 CAR circular RNA (CVB3) encodes a chimeric antigen receptor (CAR) specific for CD19. This circRNA features a scarless circularization design, eliminating linker or junction sequences between the 5′ and 3′ ends, and enables efficient circularization and expression without the need for optimized UTR elements.

This circRNA features:
- No scar sequence (exon or spacer)
- Self-circularized via the PIE strategy
- Designed with CVB3 IRES for translation initiation
- Composed entirely of unmodified nucleotides

This scarless CD19 CAR circRNA is ideal for generating CAR-expressing immune cells in preclinical research while minimizing genomic risk and maintaining simple RNA design.

产品属性

Form	Liquid
Concentration	1mg/mL
Full mRNA Molecular Weight	730168 Da
Full circRNA length	2265 nt
Storage buffer	1mM Sodium citrate, pH 6.5
Storage condition	Store at -20°C for short term (<3 months), store at -80°C for long term.

质控参数

Appearance	Clear and free of foreign particles
RNA Length	Expected size band detected
RNA Content	Target ± 5%
OD260/OD280	1.70 ~ 2.30
Endotoxin	< 10 EU/mg
pH	Target ± 0.5

应用指导

NFAT Activation Assay (Luciferase) – CD19 CAR mRNA:
In this assay, Jurkat cells were transfected with scarless CD19 CAR circular RNA (CVB3) and subsequently co-incubated with NLAM cells expressing the CD19 antigen to assess CAR-mediated signaling. Activation of the CAR triggered the NFAT signaling pathway, leading to luciferase expression under NFAT-responsive promoter control. The resulting luminescence intensity served as a quantitative measure of NFAT activation — where higher luminescence indicates stronger CAR activation and higher binding affinity between the CD19 CAR and its antigen. This assay provides a functional readout of CAR engagement and signaling potency.

图例

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.