ParTasR mRNA (Cap1, m1Ψ)

ParTasR mRNA encodes human-codon-optimized Tas nuclease from the TIGR-Tas system. Features Cap1, m1Ψ modification, X5 codon optimization, and 100A tail. Ideal for programmable DNA targeting and precision genome editing.

价格	￥1500
产品编号	RP-A00075-0.2
规格	0.2 mg 大单询价
数量

产品属性质控参数应用指导图例

Description

The GenScript ParTasR mRNA encodes human-codon-optimized Tas nuclease, an ortholog of the recently discovered TIGR-Tas RNA-guided DNA-targeting system (Tandem Interspaced Guide RNA array–TIGR-associated (Tas) protein) as described by Faure et al., Science, 2025. ParTasR enables programmable DNA targeting, providing a novel tool for genome engineering and functional genomics studies.

This mRNA features:
- A Cap 1 structure with high capping efficiency for enhanced translation
- Codon optimization via GenScript X5 algorithm for improved protein expression
- 100% substitution with N1-methyl-pseudouridine (m1Ψ) to enhance expression and reduce innate immune activation
- A 100A poly(A) tail to mimic mature mRNA

This construct provides a reliable RNA-guided DNA-targeting tool, ideal for precision genome editing and advanced DNA manipulation research.

产品属性

Form	Liquid
Concentration	1mg/mL
Full mRNA length	1374 nt
Full mRNA Molecular Weight	4.46×10^5 Da
Storage buffer	1mM Sodium citrate, pH 6.5
Storage condition	Store at -20°C for short term (<3 months), store at -80°C for long term.

质控参数

Appearance	Clear and free of foreign particles
RNA Length	Expected size band detected
RNA Content	Target ± 5%
Integrity	≥ 75%
OD260/OD280	1.70 ~ 2.30
Capping Efficiency	≥ 90%
Endotoxin	< 10 EU/mg
pH	Target ± 0.5

应用指导

1.2 µg of ParTasR mRNA and 0.7 µg of gRNA were electroporated into 200,000 HEK293T cells. After 72 hours of incubation, total genomic DNA was extracted, and an amplicon-based NGS library was prepared. Indel percentages at the target site were analyzed.

图例

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.