| ￥1500 | |
| RP-A00073-0.2 | |
|
|
|
|
|
|
|
|
|
The F-Luc mRNA encodes firefly luciferase (F-Luc), an enzyme responsible for the natural bioluminescence observed in fireflies and click beetles. Upon delivery into cells or animals, the expressed F-Luc enzyme catalyzes light emission in the presence of its substrate, luciferin. This mRNA enables quantitative, noninvasive monitoring of gene expression and delivery efficiency in vitro and in vivo.
|
| Form | Liquid |
| Concentration | 1mg/mL |
| Full mRNA length | 1914 nt |
| Full mRNA Molecular Weight | 622225 Da |
| Storage buffer | 1mM Sodium citrate, pH 6.5 |
| Storage condition | Store at -20°C for short term (<3 months), store at -80°C for long term. |
| Appearance | Clear and free of foreign particles |
| RNA Length | Expected size band detected |
| RNA Content | Target ± 5% |
| Integrity | ≥ 75% |
| OD260/OD280 | 1.70 ~ 2.30 |
| Capping Efficiency | ≥ 90% |
| Endotoxin | < 10 EU/mg |
| pH | Target ± 0.5 |
| Fluc mRNAs prepared with different cap analogues were formulated using the SM102 LNP formulation and intravenously injected into BALB/c mice at a dose of 0.3 mg/kg. Fluc expression levels were measured by whole-body imaging using the IVIS system following luciferin administration, and total luminescence signals were quantified at 8 and 24 hours post-injection. |
»
»
»
»
For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.
