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| RP-A00071-0.2 | |
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The hCCR9 mRNA encodes human C-C chemokine receptor 9 (CCR9), a G protein-coupled receptor (GPCR) expressed on lymphocytes and T cells. CCR9 plays a key role in directing immune cell migration, particularly to the small intestine and thymus, through interaction with its ligand CCL25. This mRNA serves as a valuable tool for studying immune cell trafficking, receptor signaling, and targeted delivery research.
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| Form | Liquid |
| Concentration | 1mg/mL |
| Full mRNA length | 1382 nt |
| Full mRNA Molecular Weight | 443108 Da |
| Storage buffer | 1mM Sodium citrate, pH 6.5 |
| Storage condition | Store at -20°C for short term (<3 months), store at -80°C for long term. |
| Appearance | Clear and free of foreign particles |
| RNA Length | Expected size band detected |
| RNA Content | Target ± 5% |
| Integrity | ≥ 75% |
| OD260/OD280 | 1.70 ~ 2.30 |
| Capping Efficiency | ≥ 90% |
| Endotoxin | < 10 EU/mg |
| pH | Target ± 0.5 |
| A549 cells were seeded in a 6-well plate. After overnight incubation, the cells were transfected using Lipofectamine™ MessengerMAX™ (or an equivalent reagent) with 2.5 µg of CCR9 mRNA. 24 hours post-transfection, CCR9 expression was analyzed by flow cytometry after staining with a CCR9 primary antibody and an anti-mouse IgG Fab2 Alexa Fluor® 647 secondary antibody. |
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For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.
