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| RP-A00069-0.2 | |
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The GenScript PE6g mRNA encodes the PE6g variant of the Prime Editor enzyme, evolved through phage-assisted evolution strategies to enhance editing performance in complex or structured genomic regions. This next-generation Prime Editor provides improved precision and efficiency for advanced gene editing applications.
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| Form | Liquid |
| Concentration | 1mg/mL |
| Full mRNA length | 6121 nt |
| Full mRNA Molecular Weight | 1.98×10^6 Da |
| Storage buffer | 1mM Sodium citrate, pH 6.5 |
| Storage condition | Store at -20°C for short term (<3 months), store at -80°C for long term. |
| Appearance | Clear and free of foreign particles |
| RNA Length | Expected size band detected |
| RNA Content | Target ± 5% |
| Integrity | ≥ 75% |
| OD260/OD280 | 1.70 ~ 2.30 |
| Capping Efficiency | ≥ 90% |
| Endotoxin | < 10 EU/mg |
| pH | Target ± 0.5 |
| 10,000 cells were seeded in a 96-well plate and cultured overnight. LNPs loaded with PE6g mRNA, HEK3 pegRNA, and HEK3 nicking guide RNA were then added to the culture medium at a total payload of 500 ng per well. After a 72-hour incubation, total DNA was extracted, and the target gene was PCR-amplified. T-to-A editing efficiency was analyzed by Sanger sequencing, and the data were processed using EditR software. |
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For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.
