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| RP-A00063-0.2 | |
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The PEmax mRNA expresses an optimized prime editor enzymatic protein, which further increased editing efficiency in synergy with PE4, PE5 and engineered pegRNAs (Chen P, et al. 2021, Cell 184, 5635-5652). After efficient delivery into cells or animals, PEmax mRNA can translate into protein including a nickase and reverse transcriptase function for gene editing in cells. |
| Form | Liquid |
| Concentration | 1mg/mL |
| Full mRNA length | 6661 nt |
| Full mRNA Molecular Weight | 2.16×10^6 Da |
| Storage buffer | 1mM Sodium citrate, pH 6.5 |
| Storage condition | Store at -20°C for short term (<3 months), store at -80°C for long term. |
| Appearance | Clear and free of foreign particles |
| RNA Length | Expected size band detected |
| RNA Content | Target ± 5% |
| Integrity | ≥ 75% |
| OD260/OD280 | 1.70 ~ 2.30 |
| Capping Efficiency | ≥ 90% |
| Endotoxin | < 10 EU/mg |
| pH | Target ± 0.5 |
| Transfect PEmax mRNA along with pegRNA and nicking sgRNA (optional) into cells through electroporation, LNP encapsulation or transfection reagent. After 3 days of incubation, extract the genomic DNA and perform PCR. Analyze the resulting PCR products by Sanger sequencing. |
T to A editing using PEmax system in HEK293T cells and primary T cells. X5 represents GenScript codon optimization algorithm. »
For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.
