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| RP-A00062-0.2 | |
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Prime Editing (PE) system consists of a Cas9 nickase fused with a reverse transcriptase domain and a prime editing guide RNA (pegRNA). Guided by the pegRNA, the Cas9-reverse transcriptase complex introduces a precise nick in the target DNA strand and generates the desired DNA sequence based on the reverse transcription template encoded within the pegRNA. The key advantages of the Prime Editing system include: no generation of double-stranded DNA breaks (DSBs), no requirement for a donor DNA template, and a reduced off-target editing rate. The PE2/PE3 mRNA sequence was from publication: Nelson, et al. Nat Biotechnology, 2022; 40: 402-410. |
| Form | Liquid |
| Concentration | 1mg/mL |
| Full mRNA length | 6619 nt |
| Full mRNA Molecular Weight | 2.14×10^6 Da |
| Storage buffer | 1mM Sodium citrate, pH 6.5 |
| Storage condition | Store at -20°C for short term (<3 months), store at -80°C for long term. |
| Appearance | Clear and free of foreign particles |
| RNA Length | Expected size band detected |
| RNA Content | Target ± 5% |
| Integrity | ≥ 75% |
| OD260/OD280 | 1.70 ~ 2.30 |
| Capping Efficiency | ≥ 90% |
| Endotoxin | < 10 EU/mg |
| pH | Target ± 0.5 |
| Transfect PE2/PE3 mRNA along with pegRNA and nicking sgRNA (optional) into cells through electroporation, LNP encapsulation or transfection reagent. After 3 days of incubation, extract the genomic DNA and perform PCR. Analyze the resulting PCR products by Sanger sequencing. |
T to A editing using PE3 system in HEK293T cells. »
For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.
