LbCas12a mRNA V2 (Cap1, m1Ψ)

价格	￥1500
产品编号	RP-A00060-0.2
规格	0.2 mg 1 mg 大单询价
数量

产品属性质控参数应用指导图例

Description

Cpf1 is a novel class of CRISPR-Cas DNA endonuclease. The LbCpf1/Cas12a allow efficient mutagenesis in zebrafish and Xenopus (Moreno-Mateos, et al. Nat Communications, 2017; 8: 2024). LbCpf1 increases homology-directed genome editing.

This mRNA features:
- A Cap 1 structure with high capping efficiency
- Codon optimized using GenScript X5 algorithm to enhance expression efficiency
- 100% substitution with N1-methyl-pseudouridine (m1Ψ) for improved protein expression and reduced innate immune response
- GenScript’s patented UTR to enhance translation
- A 100A poly(A) tail to mimic natural mature mRNA

产品属性

Form	Liquid
Concentration	1mg/mL
Full mRNA length	4063 nt
Full mRNA Molecular Weight	1.32×10^6 Da
Storage buffer	1mM Sodium citrate, pH 6.5
Storage condition	Store at -20°C for short term (<3 months), store at -80°C for long term.

质控参数

Appearance	Clear and free of foreign particles
RNA Length	Expected size band detected
RNA Content	Target ± 5%
Integrity	≥ 75%
OD260/OD280	1.70 ~ 2.30
Capping Efficiency	≥ 90%
Endotoxin	< 10 EU/mg
pH	Target ± 0.5

应用指导

Transfect 2 μg of LbCas12a mRNA and 0.4 μg of TRAC crRNA into 5×104 HEK293T cells using Lipofectamine™ MessengerMAX™ (or equivalent), on day 3, extract the genomic DNA and analyze data by NGS sequencing.

图例

Editing efficiency of Cas12a on TRAC target in HEK293T cells. WT represents wildtype; X5 represents GenScript codon optimization algorithm. »

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.