| ￥1500 | |
| RP-A00057-0.2 | |
|
|
|
|
|
|
|
|
|
CD19 mRNA is a transmembrane glycoprotein primarily expressed on B lymphocytes. It plays two major roles in human B cells: acting as an adaptor protein to recruit cytoplasmic signaling proteins to the membrane and working within the CD19/CD21 complex to decrease the threshold for B cell receptor signaling pathways. |
| Form | Liquid |
| Concentration | 1mg/mL |
| Full mRNA length | 1939 nt |
| Full mRNA Molecular Weight | 627740 Da |
| Storage buffer | 1mM Sodium citrate, pH 6.5 |
| Storage condition | Store at -20°C for short term (<3 months), store at -80°C for long term. |
| Appearance | Clear and free of foreign particles |
| RNA Length | Expected size band detected |
| RNA Content | Target ± 5% |
| Integrity | ≥ 75% |
| OD260/OD280 | 1.70 ~ 2.30 |
| Capping Efficiency | ≥ 90% |
| Endotoxin | < 10 EU/mg |
| pH | Target ± 0.5 |
| Transfect 0.5μg of CD19 mRNA into one well of cells in a 24-well plate with Lipofectamine™ MessengerMAX™ (or equivalent). 24-48 hours after transfection, detect the expression of CD19 protein using flow cytometry with staining by BD Pharmingen™ APC Mouse Anti-Human CD19 antibody (Catalog No:55541). |
Expression of CD19 mRNA in A549 cells. GenSmart, X4, and X5 represent GenScript mRNA codon optimized using different algorithms. »
For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.
