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| RP-A00056-0.2 | |
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tdTomato mRNA encodes for a red fluorescent protein derived from corals of the genus Discosoma. It displays excitation and emission maxima of 554 and 581 nm, respectively. tdTomato is optimized for cell and tissue imaging, with improved fluorescence intensity and stability. |
| Form | Liquid |
| Concentration | 1mg/mL |
| Full mRNA length | 1696 nt |
| Full mRNA Molecular Weight | 550032 Da |
| Storage buffer | 1mM Sodium citrate, pH 6.5 |
| Storage condition | Store at -20°C for short term (<3 months), store at -80°C for long term. |
| Appearance | Clear and free of foreign particles |
| RNA Length | Expected size band detected |
| RNA Content | Target ± 5% |
| Integrity | ≥ 75% |
| OD260/OD280 | 1.70 ~ 2.30 |
| Capping Efficiency | ≥ 90% |
| Endotoxin | < 10 EU/mg |
| pH | Target ± 0.5 |
| Transfect 0.14μg of tdTomato mRNA into one well of cells in a 96-well plate with Lipofectamine™ MessengerMAX™ (or equivalent). 24-48 hours after transfection, detect the expression of tdTomato protein by plate reader with excitation at 540nm and emission at 582nm. |
Expression of tdTomato mRNA in A549 cells. GenSmart, X4, and X5 represent GenScript mRNA codon optimized using different algorithms; ComP represents competitor P. »
For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.
