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scarless eGFP circular RNA (CVB3)

The scarless eGFP circular RNA (circRNA) expresses an enhanced green fluorescent protein (Ex: 488nm, Em: 507nm). This circRNA is generated via PIE- Self circularization and does not have scar sequence. CVB3 IRES is designed in this circular RNA for initiating the IRES dependent ribosome recruiting and translation. All bases are non-modified. EGFP circRNA can be used as fluorescence reporter sequence in RNA therapeutics and delivery system development projects.
￥2000
RP-A00051

Description

The scarless eGFP circular RNA (circRNA) expresses an enhanced green fluorescent protein, with the open reading frame sequence originally from the jellyfish, Aequorea Victoria. EGFP circRNA can be used as fluorescence reporter sequence in RNA therapeutics and delivery system development projects. The expressed protein has an excitation wavelength of 488nm, emission at 507nm.
 
 
This circRNA features: 
- No scar sequence (exon or spacer)
- Self-circularized via the PIE strategy 
- Designed with CVB3 IRES for translation initiation 
- Composed entirely of unmodified nucleotides
产品属性
Form Liquid
Concentration 1mg/mL  
Full mRNA Molecular Weight 470035  
Full circRNA length 1461nt  
Storage buffer 1mM Sodium citrate, pH6.5  
Storage condition Store at -20℃ for short term (<3 months), store at -80 ℃ for long term
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质控参数
Appearance Clear and free of foreign particles
RNA Length Expected size band detected
RNA Content Target ± 5%
OD260/OD280 1.70 ~ 2.30
Endotoxin < 10 EU/mg
pH Target ± 0.5
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应用指导
Transfect 0.1-0.2 µg of circRNA with 0.5 µL of Lipofectamine™ 2000 (or equivalent reagent) per well in a 96-well plate, following the manufacturer’s protocol. Analyze the expression 16-48 hours post-transfection using a plate reader, flow cytometer, fluorescence microscope, or confocal microscope. For equipment settings, we recommend: 485 nm excitation, 535 nm emission.
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For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.