eSpCas9 mRNA(Cap 1,5-MOU)

The eSpCas9 mRNA expresses enhanced-specificity SpCas9 protein (sequence reference: Slaymaker et al Science. 2015 Dec 1). eSpCas9 can reduce off-target effects by over 10-fold, while maintaining robust on-target genome editing efficiency. This mRNA is capped with Cap1 structure with high capping efficiency. It has 100% substituted with 5-methoxyuridine for enhanced expression and reduced immunogenicity. The mRNA has a 100A tail in its sequence, mimics a mature mRNA.

价格	￥1000 在线订购包邮(港澳台除外)
产品编号	RP-A00050-0.1
规格	0.1 mg 0.2 mg 1 mg 大单询价
数量
参考货期


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产品属性质控参数应用指导图例

Description

The eSpCas9 mRNA expresses enhanced-specificity SpCas9 protein, with sequence originally from Slaymaker et al., Science (2015 Dec 1). eSpCas9 significantly reduces off-target effects (by more than 10-fold), while maintaining high on-target genome editing efficiency.

This mRNA features:
- A Cap 1 structure with high capping efficiency
- 100% substitution with 5-methoxyuridine (5-MOU) for improved protein expression and reduced innate immune response
- GenScript’s patented UTR to enhance translation
- A 100A poly(A) tail to mimic natural mature mRNA

This construct offers high-precision genome editing with minimized off-target activity, making it ideal for CRISPR validation, therapeutic research, and functional genomics studies.

产品属性

Form	Liquid
Concentration	1mg/mL
Full mRNA length	4479nt
Full mRNA Molecular Weight	1458548
Storage buffer	1mM Sodium citrate, pH6.5
Storage condition	Store at -20^oC for short term (<3 months), store at – 80 ^oC for long term.

质控参数

Appearance	Clear and free of foreign particles
RNA Length	Expected size band detected
RNA Content	Target ± 5%
Integrity	≥ 75%
OD260/OD280	1.70 ~ 2.30
Capping Efficiency	≥ 90%
Endotoxin	< 10 EU/mg
pH	Target ± 0.5

应用指导

Transfect a total of 0.5-1.5 µg of eSpCas9 mRNA and sgRNA per well (recommended molar ratio 1:10), using a transfection reagent, electroporation, or LNP encapsulation in a 24-well plate (~2 × 10⁵ cells/well). Assess genome editing efficiency 48-72 hours post-transfection via sequencing and ICE analysis.

图例

Expression of eSpCas9 mRNA in A549 cells. The eSpCas9 expression was measured 48 hours after transfection by ELISA. »

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.