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saCas9 mRNA (Cap1, m1Ψ)

The saCas9 mRNA expresses an wild type Staphylococcus aureus cas9 enzymatic protein. This mRNA is capped with Cap1-AG structure with high capping efficiency. It has 100% substituted with N1-methylpseudouridine for enhanced expression and reduced immunogenicity. The mRNA has a 100A tail in its sequence, mimics a mature mRNA. After efficient delivery, saCas9 mRNA can translate into protein function for gene editing in cells.
￥1000
RP-A00047

Description

The saCas9 mRNA expresses an wild type Staphylococcus aureus cas9 (saCas9) enzymatic protein. After efficient delivery into cells or animals, saCas9 mRNA can translate into protein function for gene editing in cells.  
 
This mRNA features: 
- A Cap 1 structure with high capping efficiency 
- 100% substitution with N1-methyl-pseudouridine (m1Ψ) for improved protein expression and reduced innate immune response 
- GenScript’s patented UTR to enhance translation 
- A 100A poly(A) tail to mimic natural mature mRNA
产品属性
Form Liquid
Concentration 1mg/mL
Full mRNA length 3534nt
Full mRNA Molecular Weight 1.14 X 10^6 Da
Storage buffer 1mM Sodium citrate, pH6.5
Storage condition Store at -20°C for short term (<3 months), store at -80°C for long term.
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质控参数
Appearance Clear and free of foreign particles
RNA Length Expected size band detected
RNA Content Target ± 5%
Integrity ≥ 75%
OD260/OD280 1.70 ~ 2.30
Capping Efficiency ≥ 90%
Endotoxin < 10 EU/mg
pH Target ± 0.5
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应用指导
Transfect a total of 0.5-1.5 µg of saCas9 mRNA and sgRNA per well (recommended molar ratio 1:10), using a transfection reagent, electroporation, or LNP encapsulation in a 24-well plate (~2 × 10⁵ cells/well). Assess genome editing efficiency 48-72 hours post-transfection via sequencing and ICE analysis.
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For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.