Ovalbumin (OVA) mRNA (Cap1, m1Ψ)

Ovalbumin (OVA) is the major egg white protein, commonly used as an allergen and a model for serpin research. This mRNA is capped with Cap1 structure with high capping efficiency. It has 100% substituted with N1-methyl-pseudoUridine for enhanced expression and reduced immunogenicity. The mRNA has a CD33 secretory peptide build in to help secreting the expressed protein, and a 100A tail in its sequence, mimics a mature mRNA.

价格	￥1000 在线订购包邮(港澳台除外)
产品编号	RP-A00043-0.1
规格	0.1 mg 0.2 mg 1 mg 大单询价
数量
参考货期


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产品属性质控参数应用指导图例

Description

Ovalbumin is the main protein found in egg white. It is a commonly used antigen for stimulating an allergic reaction in test subjects. It is also widely used in studies of serpin structure and function in biochemistry.

This mRNA features:
- A Cap 1 structure with high capping efficiency
- 100% substitution with N1-methyl-pseudouridine (m1Ψ) for improved protein expression and reduced innate immune response
- GenScript’s patented UTR to enhance translation
- A 100A poly(A) tail to mimic natural mature mRNA
- An integrated CD33 signal peptide to facilitate secretion of the expressed protein

产品属性

Form	Liquid
Concentration	1mg/mL
Full mRNA length	1482nt
Full mRNA Molecular Weight	462888
Storage buffer	1mM Sodium citrate, pH6.5
Storage condition	Store at -20°C for short term (<3 months), store at -80°C for long term.

质控参数

Appearance	Clear and free of foreign particles
RNA Length	Expected size band detected
RNA Content	Target ± 5%
Integrity	≥ 75%
OD260/OD280	1.70 ~ 2.30
Capping Efficiency	≥ 90%
Endotoxin	< 10 EU/mg
pH	Target ± 0.5

应用指导

Transfect 0.1-0.5 ug of mRNA using 0.5 uL of Lipofectamine™ MessengerMAX™ Transfection Reagent (or equivalent) for one well of cells in a 96 well plate following manufacture’s instruction. Collect the cell supernatant after 12 to 48 hours, cell supertant was diluted 250x using PBS, and the ovalbumin secretion was measured using ELISA kit Ovalbumin (Sandwich ELISA) ELISA Kit - LS-F9540 from LS Bio.

图例

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.