Description
Ovalbumin is the main protein found in egg white. It is a commonly used antigen for stimulating an allergic reaction in test subjects. It is also widely used in studies of serpin structure and function in biochemistry.
This mRNA features:
- A Cap 1 structure with high capping efficiency
- 100% substitution with N1-methyl-pseudouridine (m1Ψ) for improved protein expression and reduced innate immune response
- GenScript’s patented UTR to enhance translation
- A 100A poly(A) tail to mimic natural mature mRNA
- An integrated CD33 signal peptide to facilitate secretion of the expressed protein |
| Form |
Liquid |
| Concentration |
1mg/mL |
| Full mRNA length |
1482nt |
| Full mRNA Molecular Weight |
462888 |
| Storage buffer |
1mM Sodium citrate, pH6.5 |
| Storage condition |
Store at -20°C for short term (<3 months), store at -80°C for long term. |
| Appearance |
Clear and free of foreign particles |
| RNA Length |
Expected size band detected |
| RNA Content |
Target ± 5% |
| Integrity |
≥ 75% |
| OD260/OD280 |
1.70 ~ 2.30 |
| Capping Efficiency |
≥ 90% |
| Endotoxin |
< 10 EU/mg |
| pH |
Target ± 0.5 |
|
Transfect 0.1-0.5 ug of mRNA using 0.5 uL of Lipofectamine™ MessengerMAX™ Transfection Reagent (or equivalent) for one well of cells in a 96 well plate following manufacture’s instruction. Collect the cell supernatant after 12 to 48 hours, cell supertant was diluted 250x using PBS, and the ovalbumin secretion was measured using ELISA kit Ovalbumin (Sandwich ELISA) ELISA Kit - LS-F9540 from LS Bio. |
For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.
