Description
mNeptune2.5 is a reengineered far red fluorescent protein derived from Entacmaea quadricolor, the mNeptune2.5 sequence was published by Chu J, Haynes RD, et al on Nat Methods. 2014 May; 11(5): pp 572-578. mNeptune2.5 protein can be excited at visible light range, > 600nm.
This mRNA features:
- A Cap 1 structure with high capping efficiency
- 100% substitution with N1-methyl-pseudouridine (m1Ψ) for improved protein expression and reduced innate immune response
- GenScript’s patented UTR to enhance translation
- A 100A poly(A) tail to mimic natural mature mRNA |
| Form |
Liquid |
| Concentration |
1mg/mL |
| Full mRNA length |
1001nt |
| Full mRNA Molecular Weight |
322181 |
| Storage buffer |
1mM Sodium citrate, pH6.5 |
| Storage condition |
Store at -20°C for short term (<3 months), store at -80°C for long term. |
| Appearance |
Clear and free of foreign particles |
| RNA Length |
Expected size band detected |
| RNA Content |
Target ± 5% |
| Integrity |
≥ 75% |
| OD260/OD280 |
1.70 ~ 2.30 |
| Capping Efficiency |
≥ 90% |
| Endotoxin |
< 10 EU/mg |
| pH |
Target ± 0.5 |
|
Transfect 0.1-0.5 ug of mRNA using 0.5 uL of Lipofectamine™ MessengerMAX™ Transfection Reagent (or equivalent) for one well of cells in a 96 well plate following manufacture’s instruction. Test the result after 12 to 48 hours using plate reader, or flow cytometry. Suggest using excitation wavelength at 540 nm, emission wavelength at 600 nm for equipment setting. |
For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.
