GenLNP-L01-FLuc mRNA (m1Ψ)

The LNP formulation loaded with firefly luciferase mRNA (m1Ψ) is prepared using generic LNP formulation with main ionizable lipid of LP01. The payload mRNA has all the U bases 100% modified with N1-methyl-pseudoUridine. F-Luc mRNA can translate into F-Luc and emit bioluminescence with Luciferin. This formulation is a good control for testing if LP01 LNP is a good potential formulation for your mRNA product under research in your in vitro and in vivo experiment model

价格	￥2000
产品编号	RP-A00029-50
规格	0.05 mg 大单询价
数量

产品属性质控参数应用指导图例

Description

The LNP formulation loaded with firefly luciferase mRNA (m1Ψ) is prepared using generic LNP formulation with main ionizable lipid of LP01.

The payload Fluc mRNA (m1Ψ) has all the U bases 100% modified with N1-methyl-pseudouridine (m1Ψ). F-Luc mRNA can translate into F-Luc and emit bioluminescence in the presence of its substrate, Luciferin. Fluc mRNA is a commonly used fluorescence reporter sequence in mRNA therapeutics and delivery system development projects.

This formulation is a good control for testing if LP01 LNP formulation is a good potential formulation for your mRNA product under research in your in vitro and in vivo experiment model

产品属性

Form	Liquid
Concentration	0.15mg/mL
Full mRNA length	1914nt
Full mRNA Molecular Weight	622225
Storage buffer	Tris-HCl/Sucrose
Storage condition	Store at -80°C for long term. Avoid freeze thaw, only thaw once before use.

质控参数

Appearance	Clear and free of foreign particles
Encapsulation Efficiency	> 85%
Endotoxin	< 4 EU/ml
Particle Size	65-125 nm
pH	7.4 ± 0.5
Polydispersity Index	< 0.2
Zeta Potential	± 15.0 mV

应用指导

1. LNP can be added directly to cells incubated in complete media
2. Cells grown and transfected in complete media (DMEM +10% FBS) has higher expression than reduced serum medium
3. For suspension cell lines, we recommend using low fluorescent background media such as Flourobrite® with 10% FBS, 1X GlutaMax® and 1X sodium pyruvate
4. For adherent cell line, we recommend replace complete media with Opti-mem right before reading the cell eGFP expression
5. For higher expression, we recommend incubate the LNP with FBS at 1:2 volume ratio (LNP:FBS) for 30 minutes at 37C before transfecting

图例

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.