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| RP-A00026 | |
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The GenLNP-S05-FLuc mRNA (m1Ψ) is a 5 component LNP loaded with Fluc mRNA (m1Ψ) has liver specific targeting effect after systemic administration.
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| Form | Liquid |
| Concentration | 0.15mg/mL |
| Full mRNA length | 1914nt |
| Full mRNA Molecular Weight | 622225 |
| Storage buffer | PBS/Sucrose |
| Storage condition | Store at -80°C for long term. Avoid freeze thaw, only thaw once before use. |
| Appearance | Clear and free of foreign particles |
| Encapsulation Efficiency | > 85% |
| Endotoxin | < 4 EU/ml |
| Particle Size | 65-125 nm |
| pH | 7.4 ± 0.5 |
| Polydispersity Index | < 0.2 |
| Zeta Potential | ± 15.0 mV |
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1. LNP can be added directly to cells incubated in complete media 2. Cells grown and transfected in complete media (DMEM +10% FBS) has higher expression than reduced serum medium 3. For suspension cell lines, we recommend using low fluorescent background media such as Flourobrite® with 10% FBS, 1X GlutaMax® and 1X sodium pyruvate 4. For adherent cell line, we recommend replace complete media with Opti-mem right before reading the cell eGFP expression 5. For higher expression, we recommend incubate the LNP with FBS at 1:2 volume ratio (LNP:FBS) for 30 minutes at 37C before transfecting |
For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.
