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F-Luc Circular RNA (CVB3)

The Firefly-Luciferase (F-Luc) circRNA expresses firefly luciferase protein, which is the enzyme for bioluminescence of fireflies and click beetles. This circRNA is generated via PIE- Self circularization and contains a small scar sequence. CVB3 IRES is designed in this circular RNA for initiating the IRES dependent ribosome recruiting and translation. All bases are non-modified. After efficient delivery into cells or animals, F-Luc circRNA can translate into F-Luc and emit bioluminescence in the presence of its substrate, Luciferin.
￥1000
RP-A00021
现货

Description

The Firefly-Luciferase (F-Luc) circRNA expresses firefly luciferase protein, which is the enzyme for bioluminescence of fireflies and click beetles. After efficient delivery into cells or animals. F-Luc circRNA can translate into F-Luc and emit bioluminescence in the presence of its substrate, Luciferin.  
 
This circRNA features: 
- Self-circularized via the PIE strategy 
- Includes a minimal scar sequence (Exon + Spacer) 
- Designed with CVB3 IRES for translation initiation 
- Composed entirely of unmodified nucleotides
产品属性
Form Liquid
Concentration 1mg/mL
Full mRNA Molecular Weight 828321
Full circRNA length 2570nt
Storage buffer 1mM Sodium citrate, pH6.5
Storage condition Store at -20°C for short term (<3 months), store at -80°C for long term.
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质控参数
Appearance Clear and free of foreign particles
RNA Length Expected size band detected
RNA Content Target ± 5%
OD260/OD280 1.70 ~ 2.30
Endotoxin < 10 EU/mg
pH Target ± 0.5
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应用指导
Transfect 0.1-0.2 µg of circRNA with 0.5 µL of Lipofectamine™ MessengerMAX™ (or equivalent reagent) per well in a 96-well plate, following the manufacturer’s instructions. Assess luciferase expression 12-48 hours post-transfection using luciferase assay system (Promega, E4030).
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For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.