GenLNP-S04-EGFP mRNA (m1Ψ) - CD34+ Cells Targeted

GenLNP-S04-EGFP mRNA (m1Ψ) is a CD34 antibody decorated LNP loaded with eGFP mRNA (m1Ψ). The LNP is formulated with main ionizable lipid of SM102 and functionalized with anti-human CD34 antibody mAb, Mouse (GenScript, Cat: CP0001) to enhance its targeting efficiency to CD34+ cells. The antibody is covalently conjugated to lipid molecule. The payload mRNA has all the U bases 100% modified with N1-methyl-pseudoUridine. This LNP formulation showed enhanced delivery of eGFP mRNA to CD34+ stem cells.

价格	￥4000
产品编号	RP-A00012-50
规格	0.05 mg 大单询价
数量

产品属性质控参数应用指导图例

Description

The GenLNP-S04-EGFP mRNA (m1Ψ) is a CD34 antibody decorated LNP loaded with eGFP mRNA (m1Ψ). It displays anti-human CD34 antibody mAb, Mouse (GenScript, Cat: CP0001) to enhance its targeting efficiency to CD34 positive cells. The antibody is covalently conjugated to lipid molecule for achieving targeting effect.

The LNP is formulated with main ionizable lipid of SM102.

The payload eGFP mRNA (m1Ψ) has all the U bases 100% modified with N1-methyl-pseudouridine (m1Ψ). This LNP formulation showed enhanced delivery of eGFP mRNA to CD34+ stem cells.

产品属性

Form	Liquid
Concentration	0.15mg/mL
Full mRNA length	980nt
Full mRNA Molecular Weight	319792
Storage buffer	PBS/Sucrose
Storage condition	Store at -80°C for long term. Avoid freeze thaw, only thaw once before use.

质控参数

Appearance	Clear and free of foreign particles
Encapsulation Efficiency	> 85%
Endotoxin	< 4 EU/ml
Particle Size	65-165 nm
pH	7.4 ± 0.5
Polydispersity Index	< 0.2
Zeta Potential	±20 mV

应用指导

1. LNP can be added directly to cells incubated in complete media
2. Cells grown and transfected in complete media (DMEM +10% FBS) has higher expression than reduced serum medium
3. For suspension cell lines, we recommend using low fluorescent background media such as Flourobrite® with 10% FBS, 1X GlutaMax® and 1X sodium pyruvate
4. For adherent cell line, we recommend replace complete media with Opti-mem right before reading the cell eGFP expression
5. For higher expression, we recommend incubate the LNP with FBS at 1:2 volume ratio (LNP:FBS) for 30 minutes at 37C before transfecting

图例

GenLNP-S04-EGFP MRNA (m1Ψ) - CD34+ Cells Targeted

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.