Description
The GenLNP-S03-EGFP mRNA (m1Ψ) is a CD3 antibody decorated LNP loaded with eGFP mRNA (m1Ψ) displays anti-human CD3 antibody (OKT3) mAb, Mouse (GenScript, Cat: A02199) to enhance its targeting efficiency to CD3 positive cells. The antibody is covalently conjugated to lipid molecule for achieving targeting effect.
The LNP is formulated with main ionizable lipid of SM102.
The payload eGFP mRNA (m1Ψ) has all the U bases 100% modified with N1-methyl-pseudouridine (m1Ψ). This LNP formulation showed enhanced delivery of eGFP mRNA to CD3+ T cells. |
| Form |
Liquid |
| Concentration |
0.15mg/mL |
| Full mRNA length |
980nt |
| Full mRNA Molecular Weight |
319792 |
| Storage buffer |
PBS/Sucrose |
| Storage condition |
Store at -80°C for long term. Avoid freeze thaw, only thaw once before use. |
| Appearance |
Clear and free of foreign particles |
| Encapsulation Efficiency |
> 85% |
| Endotoxin |
< 4 EU/ml |
| Particle Size |
65-165 nm |
| pH |
7.4 ± 0.5 |
| Polydispersity Index |
< 0.2 |
| Zeta Potential |
±20 mV |
1. LNP can be added directly to cells incubated in complete media
2. Cells grown and transfected in complete media (DMEM +10% FBS) has higher expression than reduced serum medium
3. For suspension cell lines, we recommend using low fluorescent background media such as Flourobrite® with 10% FBS, 1X GlutaMax® and 1X sodium pyruvate
4. For adherent cell line, we recommend replace complete media with Opti-mem right before reading the cell eGFP expression
5. For higher expression, we recommend incubate the LNP with FBS at 1:2 volume ratio (LNP:FBS) for 30 minutes at 37C before transfecting |
For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.
