GenLNP-A01-EGFP mRNA (m1Ψ)

价格	￥2000 在线订购包邮(港澳台除外)
产品编号	RP-A00010-50
规格	0.05 mg 大单询价
数量
参考货期


咨询产品问题

产品属性质控参数应用指导图例

Description

The LNP formulation loaded with eGFP mRNA (m1Ψ) is prepared using generic LNP formulation with main ionizable lipid of ALC0315.

The payload EGFP mRNA (m1Ψ) has all the U bases 100% modified with N1-methyl-pseudouridine (m1Ψ). It expresses an enhanced green fluorescent protein, with the open reading frame sequence originally from the jellyfish, Aequorea Victoria. EGFP mRNA is a commonly used fluorescence reporter sequence in mRNA therapeutics and delivery system development projects. The expressed protein has an excitation wavelength of 488nm, emission at 507nm.

This formulation is a good control for testing if ALC0315 LNP formulation is a good potential formulation for your mRNA product under research in your in vitro and in vivo experiment model.

产品属性

Form	Liquid
Concentration	0.15mg/mL
Full mRNA length	980nt
Full mRNA Molecular Weight	319792
Storage buffer	Tris-HCl/Sucrose
Storage condition	Store at -80°C for long term. Avoid freeze thaw, only thaw once before use.

质控参数

Appearance	Clear and free of foreign particles
Encapsulation Efficiency	> 85%
Endotoxin	< 4 EU/ml
Particle Size	50-100 nm
pH	7.4 ± 0.5
Polydispersity Index	< 0.2
Zeta Potential	± 15.0 mV

应用指导

1. LNP can be added directly to cells incubated in complete media
2. Cells grown and transfected in complete media (DMEM +10% FBS) has higher expression than reduced serum medium
3. For suspension cell lines, we recommend using low fluorescent background media such as Flourobrite® with 10% FBS, 1X GlutaMax® and 1X sodium pyruvate
4. For adherent cell line, we recommend replace complete media with Opti-mem right before reading the cell eGFP expression
5. For higher expression, we recommend incubate the LNP with FBS at 1:2 volume ratio (LNP:FBS) for 30 minutes at 37C before transfecting

图例

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.