Description
Cre is a tyrosine recombinase enzyme derived from the P1 bacteriophage. The cre-loxP-mediated recombination system is an integral experimental tool for mammalian genetic and cell biology.
This mRNA features:
- A Cap 1 structure with high capping efficiency
- 100% substitution with N1-methyl-pseudouridine (m1Ψ) for improved protein expression and reduced innate immune response
- GenScript’s patented UTR to enhance translation
- A 100A poly(A) tail to mimic natural mature mRNA |
| Form |
Liquid |
| Concentration |
1mg/mL |
| Full mRNA length |
1407nt |
| Full mRNA Molecular Weight |
459549 |
| Storage buffer |
1mM Sodium citrate, pH6.5 |
| Storage condition |
Store at -20°C for short term (<3 months), store at -80°C for long term. |
| Appearance |
Clear and free of foreign particles |
| RNA Length |
Expected size band detected |
| RNA Content |
Target ± 5% |
| Integrity |
≥ 75% |
| OD260/OD280 |
1.70 ~ 2.30 |
| Capping Efficiency |
≥ 90% |
| Endotoxin |
< 10 EU/mg |
| pH |
Target ± 0.5 |
|
Transfect 0.1 ug of mRNA using 0.5 uL of Lipofectamine™ MessengerMAX™ Transfection Reagent (or equivalent) for one well of HEK293-LoxP-GFP-RFP(Bsd) cells in a 96 well plate following manufacture’s instruction. Detect the RFP expression on cells using plate reader at the excitation of 560nm, and emission at 620nm. |
For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.
