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Cre mRNA (Cap1, m1Ψ)

Cre is a tyrosine recombinase enzyme derived from the P1 bacteriophage. The cre-loxP-mediated recombination system is an integral experimental tool for mammalian genetic and cell biology. This mRNA is capped with Cap1 structure with high capping efficiency. It has 100% substituted with N1-methyl-pseudoUridine for enhanced expression and reduced immunogenicity. The mRNA has a 100A tail in its sequence, mimics a mature mRNA.
RP-A00005
¥1000

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Description

Cre is a tyrosine recombinase enzyme derived from the P1 bacteriophage. The cre-loxP-mediated recombination system is an integral experimental tool for mammalian genetic and cell biology.  
 
This mRNA features: 
- A Cap 1 structure with high capping efficiency 
- 100% substitution with N1-methyl-pseudouridine (m1Ψ) for improved protein expression and reduced innate immune response 
- GenScript’s patented UTR to enhance translation 
- A 100A poly(A) tail to mimic natural mature mRNA

Form Liquid
Concentration 1mg/mL
Full mRNA length 1407nt
Full mRNA Molecular Weight 459549
Storage buffer 1mM Sodium citrate, pH6.5
Storage condition Store at -20°C for short term (<3 months), store at -80°C for long term.

Appearance Clear and free of foreign particles
RNA Length Expected size band detected
RNA Content Target ± 5%
Integrity ≥ 75%
OD260/OD280 1.70 ~ 2.30
Capping Efficiency ≥ 90%
Endotoxin < 10 EU/mg
pH Target ± 0.5

Transfect 0.1 ug of mRNA using 0.5 uL of Lipofectamine™ MessengerMAX™ Transfection Reagent (or equivalent) for one well of HEK293-LoxP-GFP-RFP(Bsd) cells in a 96 well plate following manufacture’s instruction. Detect the RFP expression on cells using plate reader at the excitation of 560nm, and emission at 620nm.

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.


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