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| RD00887 | |
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| Product Description | GS-J2C/CD16A-158F cells is possesses the 158F variant of the FcγRIIIa receptor and specific for FcγRIIIa-mediated (158F) ADCC. |
| Culture Properties | Suspension |
| Product Type | Cell line |
| Size | 2 vials of frozen cells (>3X106 per vial in 1 ml) |
| Storage | Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received. |
| Culture Medium | RPMI 1640, 10% FBS, 1 μg/mL Puromycin, 400 μg/mL Hygromycin B |
| Complete Growth Medium | RPMI 1640, 10% FBS |
| Freeze Medium-DATA | 90% Complete Growth Medium, 10% DMSO |
Anti-CD20 antibody was added in the culture of Raji (CD20+) target cells, followed by addition of FcγRIIIa effector cells. After 6-hour incubation, luciferase substrate reagent was added and luminescence signal measured by PHERAStar FSX. Data were analyzed by GraphPad Prism® software. »
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
