| 询价 | |
| M00562 | |
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| Product Description | Recombinant HEK293 cells stably overexpress human glucagon-like peptide-1 (GLP1R) on the surface and contain high levels of G protein Gαs to couple with the receptor in downstream signaling pathways. |
| Culture Properties | Adherent. |
| Stability | Stable through more than 16 passages with no significant changes in assay performance or expression profile. |
| Size | Two vials of frozen cells (>1×106 per vial in 1 mL) |
| Storage | Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received. |
| Culture Medium | DMEM, 10% FBS, 400 μg/ml Geneticin (Cat. No. 10131-035, Life Technologies), 100 μg/ml Hygromycin B (Cat. No. 10687010, Life Technologies) |
| Complete Growth Medium | DMEM (Cat. No. 10566, Life Technologies), 10% FBS. |
| Freeze Medium-DATA | 95% FBS (Cat. No. 10099-141, Life Technologies), 5% DMSO (Cat. No. D2650, Sigma) |
Figure 1. GLP-1 (7-37)-induced luciferase expression in HEK293/CRE-Luc/GLP1R cells. After stimulation by agonist GLP-1 (7-37), the cells are determined with Fire-Lumi™ Luciferase Assay System (Cat. No. L00877C-100, Genscript) and the relative luminescence units (RLU) were recorded by plate reader (Pherastar, BMG). The RLU were plotted against the log of the cumulative doses of GLP-1 (7-37) (Mean ± SEM, n = 3). The EC50 of GLP-1 (7-37) on cells was 0.29 nM.
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Figure 2. Dose dependent stimulation of intracellular cAMP accumulation upon treatment with GLP-1 (7-37) in HEK293/CRE-Luc/GLP1R cells. d2 acceptor fluorophore-labeled cAMP (Cat. No. 62AM4PEC; Revvity) and intracellular cAMP in HEK293/CRE-Luc/GLP1R cells competitively bind with Europium Cryptate-labeled anti-cAMP monoclonal antibody. The FRET signal decreases as the intracellular cAMP concentration rises and was measured by plate reader (Varioskan, ThermoFisher). The EC50 of GLP-1 (7-37) on cells was 0.29 nM. »
Figure 3. FACS analysis of cell surface expression of human GLP1R on HEK293/CRE-Luc/GLP1R cells. The HEK293/CRE-Luc/GLP1R cells (blue) and the negative control HEK293 cells (red) were probed using GLP-1-Fc fusion protein (GenScript) and FITC-conjugated goat anti-human IgG (H+L) (Cat. No. A01318, Genscript). »
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
