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cGMP ELISA Detection Kit

GenScript cGMP ELISA Detection Kit is a competition enzyme-linked immunoassay which can be used for quantitative detection of cGMP in samples such as serum, plasma, saliva, cell culture supernatant, and urine.
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L00461

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Product Description Guanosine 3’, 5’‐cyclic monophosphate (cyclic GMP; cGMP) is an important secondary messenger in signal transduction pathways. It is a common regulator of ion channel conductance, glycogenolysis, and cellular apoptosis. cGMP is a cyclic nucleotide derived from guanosine triphosphate (GTP). The cellular production of cGMP is mediated by Guanylate cyclase (GC). It activates the cGMP-dependent protein kinases which in turn phosphorylate several downstream protein targets. cGMP has an effect on the regulation of cAMP levels by activating or inhibiting specific phosphodiesterases (PDEs).
GenScript cGMP ELISA Detection Kit is a competition enzyme-linked immunoassay which can be used for quantitative detection of cGMP in samples such as serum, plasma, saliva, cell culture supernatant, and urine. The anti-IgG Capture Plate is pre-coated with fixed amount of Goat anti-rabbit IgG to capture Rabbit Anti-cGMP Polyclonal Antibody. When free cGMP or specimen and HRP-cGMP conjugate are added to the well, they compete in the solution to interact with the cGMP antibody captured on the plate. Other unbound molecules are removed by a wash step. The cGMP-HRP reacts with TMB Solution to develop a blue product in the solution.
The reaction is stopped by adding stop solution and the color turns yellow which can be read at 450 nm by a Microtiter plate reader. Becuase the concentration of cGMP-HRP is held constant in the assay, the intensity of color is inversely proportional to the cGMP concentration in the sample and standards. Using the standard curve, the amount of cGMP present in the unknown samples can be calculated by transforming its absorbance value.
Detection Range 0.3-66.7 pmol/ml
Sensitivity 0.11 pmol/ml
Test Samples Serum, plasma, saliva, cell culture supernatant, and urine
Conveniency All reagents and buffers for test are provided
Complete the test within 2.5 hours
Storage The unopened kit is stable for 12 months if stored at 2-8 °C, and the opened kit is stable for 2 weeks at 2-8 °C. Do not freeze the kit.
Kit Contents
Component Quantity Part. No
Anti-IgG Capture Plate 1 plate (8 wells x 12 strips) 461-80
Anti-cGMP pAb 12 ml 461-20
HRP-cGMP 6 ml 461-30
cGMP Standards (0, 0.3, 0.8, 2.5, 7.4, 22.2, 66.7 pmol/ml) 1.5 ml 461-11,12,13,14,15,16,17
cGMP Standard Stock (10 nmol/ml) 500 μl 461-10
Assay Buffer A 60 ml 461-60
Assay Buffer B 1 ml 461-90
20× Wash Solution 40 ml B-A1-70
TMB Solution 12 ml B-A1-40
Stop Solution 6 ml B-A1-50
Plate Sealer 2 pieces N/A
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CGMP ELISA Detection Kit

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CGMP ELISA Detection Kit

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For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.