|Description||GenScript M-MuLV Reverse Transcriptase (M-MLV) synthesizes a complementary cDNA strand initiating from a primer using RNA as a template (cDNA synthesis), making it ideal for a wide range of applications.
M-MMLV is derived from a cloned region of the pol gene of M-MLV and isolated from an E. coli strain overexpressing this construct. To increase cDNA yields and get a higher percentage of longer transcripts, the M-MLV Reverse Transcriptase has been modified with reduced RNase H activity, and expressed free of exogenous RNases and other nucleases.
|Reaction Buffer||250 mM Tris-HCl (pH 8.3 at room temperature), 375 mM KCl, 15 mM MgCl₂|
|Storage||M-MuLV Reverse Transcriptase is supplied with 1x storage buffer (20 mM Tris-HCl, 100 mM NaCl, 0.01% NP-40, 0.1 mM EDTA, 1 mM DTT, 50% Glycerol pH 7.5 at 25°C). The recommended storage temperature is -20°C. Guaranteed stable for 12 months when stored properly.|
|Note||One unit is defined as the amount of enzyme required to catalyze the transfer of 1 nmol of deoxynucleotide into acid-precipitable material in 10 minutes at 37°C.|
Competitive performance of M-MLV Reverse Transcriptase.
A 0.5 kb RT−PCR product is obtained from 100 ng of total mouse liver RNA using β−actin primers. Compared with competitors, GenScript M-MLV displays efficient amplification performance. M: Marker; lane 1: 200 U M-MLV from competitor; lane 2: 500 U GenScript M-MLV; lane 3: 200 U GenScript M-MLV; lane 4: 100 U GenScript M-MLV.