| ￥1980 在线订购包邮(港澳台除外) | |
| A02002-40 | |
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| Specificity | MonoRab™ HA tag Antibody recognizes HA tags placed at N-terminal, C-terminal, and internal regions of fusion proteins. |
| Host Species | Rabbit |
| Immunogen | A synthetic peptide containing the influenza hemagglutinin epitope (YPYDVPDYA). conjugated to KLH |
| Conjugate | Biotin |
| Working concentrations for specific applications should be determined by the investigators. The appropriate concentration may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product. | |||||||
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| Form | Lyophilized |
| Storage Buffer | Lyophilized with PBS, pH 7.4, contains 1% BSA and 0.02% sodium azide. |
| Reconstitution | Reconstitute the lyophilized powder with deionized water (or equivalent) to a final concentration of 0.5 mg/ml. |
| Storage Instructions | The lyophilized product remains stable for 1 year at -20℃ from the date of receipt. Upon reconstitution, it can be stored for 2-3 weeks at 2-8℃ or for 12 months at -20℃ or below. Avoid repeated freezing and thawing cycles. |
| Purification | Protein A affinity column |
| Isotype | Rabbit IgG |
| Clonality | Monoclonal |
| Clone ID | 109B2 |
| Note | GenScript can customize this product per customer's request including product size, buffer components, etc. |
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Western blot analysis of MonoRab™ HA tag Antibody (109B2) [Biotin], mAb, Rabbit with HA-tag fusion proteins.
Loading:
Lane 1 : 25 ng N-terminal HA-tagged fusion protein
Lane 2 : 10 ng N-terminal HA-tagged fusion protein
Lane 3 : 5 ng N-terminal HA-tagged fusion protein
Lane 4 : 1 ng N-terminal HA-tagged fusion protein
Lane 5: 25 ng C-terminal HA-tagged fusion protein
Lane 6: 10 ng C-terminal HA-tagged fusion protein
Lane 7: 5 ng C-terminal HA-tagged fusion protein
Lane 8: 1 ng C-terminal HA-tagged fusion protein
Primary Antibody:
MonoRabᵀᴹ HA tag Antibody (109B2)[Biotin], mAb, Rabbit (A02002-40) 1 μg/ml
Secondary Antibody:
Streptavidin-HRP 0.2 μg/ml
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| Target Background | The rabbit immune system generates antibody diversity and optimizes affinity. GenScript utilizes MonoRab™ technology to generate high affinity and specific monoclonal rabbit antibodies. The HA tag is composed of several amino acids containing YPYDVPDYA and does not appear to interfere with the bioactivity or the biodistribution of recombinant proteins.GenScript MonoRab™ HA tag Antibody (109B2) [Biotin], mAb, Rabbit is the biotin conjugated form of MonoRab™ HA tag Antibody (109B2), mAb, Rabbit. This antibody is suitable for detecting the expression level of HA tag fusion proteins. |
| Synonyms | Rabbit monoclonal to HA tag |
For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.
