|Synonyms||Rabbit Monoclonal to Avastin|
|Description||MonoRabᵀᴹ Anti-Bevacizumab Antibody (30E1), mAb, Rabbit|
|Purification||Protein A affinity column|
|Concentration||0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide. (It may be customized for bulk orders.)|
|Reconstitution||Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final concentration of 0.5 mg/ml.|
|Specificity||The product is specific for Bevacizumab but can't block vascular endothelial growth factor 165 (VEGF 165). The antibody is recommended as a capture antibody in a pharmacokinetic (PK) bridging assay with detection antibody GenScript, A01896-40, MonoRabTM Anti- Bevacizumab Antibody (46E3)[Biotin], mAb, Rabbit.|
|Storage||The lyophilized product remains stable up to 1 year at -20 °C from date of receipt. Upon reconstitution, it can be stored for 2-3 weeks at 2-8 °C or for up to 12 months at -20 °C or below. Avoid repeated freezing and thawing cycles.|
|Note||GenScript can customize this product per customer's request including product size, buffer components, etc.|
|Working concentrations for specific applications should be determined by the investigators. The appropriate concentration may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA detection: 0.01-0.1 µg/ml
Other applications: user-optimized
MonoRabᵀᴹ Anti-Bevacizumab Antibody (30E1), mAb, Rabbit (GenScript, A01895-40) binds with Bevacizumab. While the antibody does not recognize the human IgG Fc fragment (data not shown).
Coating antigen: Bevacizumab, 1 µg/ml.
Anti-Bevacizumab antibody (GenScript, A01895-40) dilution start from 2,000 ng/ml.
EC₅₀= 7.280 ng/ml.