THE™ NWSHPQFEK Tag Antibody,mAb,Mouse
THE™ NWSHPQFEK Tag Antibody, mAb, Mouse recognizes NWSHPQFEK tags localized at the N-terminal
and C-terminal region of Strep II-tagged fusion proteins.
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| A01732 | |
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| ￥2500 | |
| A01732 | |
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| Specificity |
THE™
NWSHPQFEK Tag Antibody, mAb, Mouse recognizes NWSHPQFEK tags localized at the N-terminal
and C-terminal region of Strep II-tagged fusion proteins. |
| Host Species | Mouse |
| Immunogen | NWSHPQFEK peptide conjugated to KLH |
| Conjugate | Unconjugated |
| Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product. | |||||||||||||
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| Form | Lyophilized |
| Storage Buffer |
Lyophilized
with PBS, pH 7.4, containing 0.02% sodium azide. |
| Reconstitution |
Reconstitute
the lyophilized powder with deionized water (or equivalent) to a final
concentration of 0.5 mg/mL. |
| Storage Instructions |
The
lyophilized product remains stable for up to 1 year at -20°C from the date of
receipt. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For
long term storage, aliquot and store at -20°C or below. Avoid repeated freezing
and thawing cycles. |
| Purification | Protein A affinity column |
| Isotype | Mouse IgG1,κ |
| Clonality | Monoclonal |
| Clone ID | 5A9F9 |
| Note |
GenScript
can customize this product per customer's request including product size,
buffer components, etc. |
»
Figure 5. The affinity of THE™ NWSHPQFEK Tag Antibody, mAb, Mouse (GenScript, A01732) for N-terminal Strep II in an N-terminal Strep II tagged fusion was analyzed by Western blotting using THE™ NWSHPQFEK Tag Antibody, mAb, Mouse (GenScript, A01732, 0.2 µg/mL). The signal was developed with IRDye™ 800 Conjugated affinity Purified Goat Anti-Mouse IgG. »
Figure 1. The affinity of THE™ NWSHPQFEK Tag Antibody, mAb, Mouse (GenScript, A01732) for Strep II was analyzed in a flow cytometric analysis of non-transfected or Strep II gene-transfected CHO cells (black and blue, respectively) using THE™ NWSHPQFEK Tag Antibody, mAb, Mouse (GenScript, A01732). The signal was developed with FITC conjugated Goat Anti-Mouse IgG. »
Figure 4. The affinity of THE™ NWSHPQFEK Tag Antibody, mAb, Mouse (GenScript, A01732) for Strep II in immunoprecipitates was analyzed using Western blotting. Immunoprecipitates from Strep II-tagged protein-transfected HEK293 cell lysates using THE™ NWSHPQFEK Tag Antibody, mAb, Mouse (GenScript, A01732).
Lane 1: Strep II tagged protein transfected HEK293 cell lysates as input control.
Lane 2: Immunoprecipitates of NWSHPQFEK-tagged protein-transfected HEK293 cell lysates with THE™ NWSHPQFEK Tag Antibody, mAb, Mouse (GenScript, A01732).
Lane 3: Immunoprecipitates of the non-transfected HEK293 cell lysates with THE™ NWSHPQFEK Tag Antibody, mAb, Mouse (GenScript, A01732).
»
Figure 3. The affinity of THE™ NWSHPQFEK Tag Antibody, mAb, Mouse (GenScript, A01732) for Strep II was analyzed in a Western blot analysis of C-terminal Strep II tagged fusion protein using THE™ NWSHPQFEK Tag Antibody, mAb, Mouse (GenScript, A01732, 0.2 µg/mL). The signal was developed with IRDye™ 800 Conjugated affinity Purified Goat Anti-Mouse IgG. »
Figure 2. The affinity of THE™ NWSHPQFEK Tag Antibody, mAb, Mouse (GenScript, A01732) for Strep II was analyzed in an immunocytochemical/immunofluorescence analysis of Strep II tagged protein-transfected HEK293 cells using THE™ NWSHPQFEK Tag Antibody, mAb, Mouse (Cat.No. A01732) and Mouse IgG Control (Whole Molecule), Purified (GenScript, A01007). The signal was developed with FITC conjugated Goat Anti-Mouse IgG. »
| Target Background |
Streptavidin
is a tetrameric protein purified from Streptomyces
avidinii. It is widely used in numerous molecular biological protocols due
to its strong affinity for biotin. NWSHPQFEK (Strep tag II) is a nine amino
acid peptide with high specificity and affinity towards streptavidin. NWSHPQFEK
Tag Antibody is a useful tool in analysis and affinity purification of Strep
tag II fusion proteins. |
| Synonyms |
THE™
NWSHPQFEK Tag Antibody,mAb,Mouse |
For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.
