sELISA; WB;

Mouse IgG1,κ

Biotin

purified recombinant Protein A

Human

HEK293

TIGIT (Met22-Pro141)_x000D_ Accession # Q495A1-1	hFc
N-term	C-term

TIGIT; VSIG9; VSTM3; WUCAM

Mouse

HEK293

TIGIT (Gly26-Thr143)_x000D_ Accession # NP_001139797.1	hFc
N-term	C-term

TIGIT; VSIG9; VSTM3; WUCAM

Human

HEK 293

Mesothelin (Glu296-Gly580) Accession # Q13421	hFc
N-term	C-term

MSLN; CAK1 antigen; CAK1; megakaryocyte potentiating factor; Mesothelin; MPF; MPFSMRP; Pre-pro-megakaryocyte-potentiating factor; SMR; soluble MPF mesothelin related protein

GenScript’s Protein A Resin FF is an affinity chromatography media designed for easy, one-step purification of classes, subclasses and fragments of immunoglobulins from biological fluids and from cell culture media. Protein A Resin FF can also be used for immunoprecipitation of proteins, protein complexes or antigens.The recombinant protein A ligand is coupled to 4% highly cross-linked agarose. The coupling is optimized to have a high binding capacity for immunoglobulins. The static binding capacity of Protein A Resin FF is greater than 40 mg human IgG/ ml settled resin. The dynamic binding capacity will vary depending on several factors such as target antibody, flow rate, etc. Table 1 lists the characteristics of Protein A Resin FF.Protein A, a bacterial cell wall protein isolated from Staphylococcus aureus, binds to mammalian IgGs mainly at their Fc region. Native protein A has five IgG binding domains and many other domains with unknown functions. Recombinant protein A contains five high affinity IgG binding domains with other non-essential domains removed to reduce nonspecific binding. Since only the Fc region is involved in binding to recombinant protein A, the Fab region is available for binding antigens.

Human

E.coli

CARHSP1 (Ser2-Ser147) Accession # Q9Y2V2

CRHSP-24; CRHSP24; CSDC1

Human

HEK293

Avi	His	FGL2 (Val205-Pro439) Accession # Q14314
N-term		C-term

Biotin

Human

HEK 293

Amphiregulin (Ser101-Lys198) Accession # Q5U026

AR; AREG; Colorectum cell-derived growth factor (CRDGF)

Mouse

HEK293

Avi	His	FGL2 (Pro197-Pro432)_x000D_ Accession # P12804
N-term		C-term

Fibroleukin; pT49; FGL2; fibrinogen-like 2;

SARS-CoV-2

Sf9 insect cells

Spike ECD (Val16-Pro1213) Accession # P0DTC2	Poly-His	Flag
N-term		C-term

S protein; S-ECD; ECD protein; ECD; 2019-nCoV ECD