Liu D's Publication in J Exp Med...staining, splenocytes were stimulated with 10 nM OVA257-264 (SIINFEKL) or 10 microM OVA323-339 (ISQAVHAAHAEINEAGR; GenScript, Inc.) where indicated, in the presence of 10 microg/ml Brefeldin A for 4 h. An intracellular
Dennis J. van de Wijngaart's Publication in Prostate... Netherlands). The (ARE) 2 TATA-Luc reporter construct has previously been described as (PRE) 2 -E1b-Luc 47. Cell-Penetrating Peptides. Cell-penetrating peptides were purchased from GenScript Corp.
Cancer Cell.2020-03; Sagnella SM, Yang L, Stubbs GE, Boslem E, Martino-Echarri E, Smolarczyk K, Pattison SL, Vanegas N, St Clair E, Clarke S, Boockvar J, MacDiarmid JA, Brahmbhatt H
KBY Elbaradie's publication in Biochim Biophys Acta... Island, NY). In order to lower the background in the images, a ONE-HOUR IP-Western Kit (<Genscript>) was used. The immunoprecipitated samples were then subjected to Western blot analysis. 2.3.4. Rec
Joyner C's Publication in PLoS One...Tissues were incubated overnight at room temperature with custom primary polyclonal antibodies (GenScript, Piscataway, New Jersey, USA) diluted in StartingBlock™ T20 Blocking Buffer (Thermoscientific) at th
