| Products/Services Used | Details | Operation |
|---|---|---|
| Protein and Antibody Isolation | Protein expression was induced with 1 mM IPTG (Solarbio, China) at 18 °C for 16 h to enhance soluble protein yield. Cells were harvested by centrifugation, resuspended in lysis buffer (500 mM NaCl, 50 mM Tris-HCl, pH 8.0, 5% glycerol), and lysed via sonication. The supernatant was purified using Ni-NTA resin (GenScript Biotech, China) to obtain the protein monomer. | Get A Quote |
Brucellosis, amajor global zoonosis causingover 2.1 millionhumaninfectionsannually,posesasignificant threattopublic healthandlivestockeconomies.Conventionalvaccinesfacesubstantial limitations, includingresidualvirulenceanddiagnosticinterferencein liveattenuatedvaccines,andpoorimmunogenicityinsubunitvaccines. To address these issues, we developed BP26-OMP16Es, a self assemblingnanoparticlevaccineconstructedby fusing immunodomi nant B-andT-cell epitopes fromOMP16 to the self-assembling antigen BP26 via flexible linkers, yielding single-copy (1×) and double-copy (2×) epitope tandem nanoparticles. These particles formed homogeneous, barrel-shaped structures (14−23 nm) with strongimmunoreactivityagai... More
