| Products/Services Used | Details | Operation |
|---|---|---|
| Peptide Synthesis | HDL-C and LDL-C assay kits were obtained from the Nanjing Jiancheng Bioengineering Institute (Nanjing, China). The abalone peptides were synthesized by GenScript (Nanjing, China). 2.2 Enzymatic digestion Pepsin, acid protease, flavourzyme, papain, and alkaline protease were used to hydrolyze abalone viscera, respectively. peptides on the micelle solubility of cholesterol After sequence identification, the peptide sequences were obtained through solid-phase synthesis by GenScript (Nanjing, China). The inhibitory effect of the peptide on cholesterol micelle solubility was measured as previously described with some modifications binding interactions with the pockets (i.e., those with scores ranked close to the top) were selected for solid-phase synthesis, which was carried out at GenScript, to obtain the desired lipid-lowering peptides. The ADMET prediction was conducted as per the following steps: (1) Create a 2D structure library of peptides | Get A Quote |
Introduction Hyperlipidemia remains a major disease threatening global public health. The morbidity and mortality associated with cardiovascular diseases have been increasing. The inhibition of 3-Hydroxy-3-methylglutaryl-coenzyme A reductase (HMGCR), a key enzyme in the cholesterol synthesis pathway, can effectively reduce cholesterol levels.Methods and results In this study, the most suitable protease for preparing HMGCR inhibitory peptides was screened using the evaluation indexes of peptide yield and HMGCR inhibition rate. Peptide sequences with molecular weights <1 kDa were identified, and peptide fragments were docked with HMGCR for virtual screening. The inhibitory effects of these peptides on HMGCR activ... More
