| Products/Services Used | Details | Operation |
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| Endotoxin Detection & Removal System | collected. This process was repeated 8 times. The final LPS level of the protein (<0.1 EU/ g) was examined with LAL test (ToxinSensor gel clot assay, Genscript). The purified ST-E2 was characterized by DLS (Zetasizer Nano ZS, Malvern), SDS-PAGE, and bicinchoninic acid assay (BCA) as previously described (Molino Amebocyte Lysate (LAL) test was used to measure the endotoxin level (<0.1 EU/ g). CBU1910 used for vaccination and IgG detection by ELISA was obtained from Genscript (Piscataway, NJ); CBU1910 was expressed in E. coli BL21 cells and purified by column chromatography, and endotoxin was removed. The purified CBU1910 | Get A Quote |
AbstractQ fever is a zoonotic infectious disease caused by Coxiella burnetii, and there is currently no FDA-approved vaccine for human use. The whole-cell inactivated vaccine Q-VAX, which is only licensed in Australia, has a risk of causing severe adverse reactions, making subunit vaccines a good alternative. However, most subunit antigens are weak immunogens and require two or more immunizations to elicit an adequate level of immunity. We hypothesized that by combining a nanoparticle to co-deliver both a protein antigen and an adjuvant, together with a hydrogel depot for sustained-release kinetics, a single-administration of a nanoparticle-loaded hydrogel vaccine could elicit a strong and durable immune respon... More
