| Products/Services Used | Details | Operation |
|---|---|---|
| Peptide Synthesis | through a 0.2 m nylon syringe filter (Fisher Scientific). Matrix metalloproteinase (MMP) analog such as Tryp-MMP with amino acid sequence KCGGPQGIWGQGCK (Genscript) was used to prepare MMP degradable PEG hydrogels. Tryp-MMP was resuspended in 1 PBS at a stock of 200 mM and sterile-filtered through a 0.2 m nylon at a stock of 200 mM and sterile-filtered through a 0.2 m nylon syringe filter (Fisher Scientific). CRGDS (5 C-terminal modification of amidation; Genscript) peptide was incorporated for cell adhesion. CRGDS was resuspended in 1 PBS at a concentration of 200 nM and sterile-filtered through a 0.2 m nylon | Get A Quote |
The tailorable properties of synthetic polyethylene glycol (PEG) hydrogels make them an attractive substrate for human organoid assembly. Here, we formed human neural organoids from iPSC-derived progenitor cells in two distinct formats: (i) cells seeded on a Matrigel surface; and (ii) cells seeded on a synthetic PEG hydrogel surface. Tissue assembly on synthetic PEG hydrogels resulted in three dimensional (3D) planar neural organoids with greater neuronal diversity, greater expression of neurovascular and neuroinflammatory genes, and reduced variability when compared with tissues assembled upon Matrigel. Further, our 3D human tissue assembly approach occurred in an open cell culture format and created a tissue ... More
