| Products/Services Used | Details | Operation |
|---|---|---|
| Recombinant Proteins | (ThermoFisher, cat 13778150) for 72 h post-transfection at 37 C, 5 % CO 2 . Three replicates per cell line were performed. All siRNAs were purchased from GENScript and used at a final concentration of 10 nM. siRNA sequences are listed in the following table ( Table 1 ). Table 1 The siRNA sequences. siRNA Sense strand lysate concentration was quantified using PIERCE BCA PROTEIN ASSAY KIT (Invitrogen, cat#23225, USA). The proteins were separated by SDS-PAGE on 4-20% gel (Genscript, cat# M42012C) and transferred to nitrocellulose membranes (Invitrogen, cat# IB23001). The membranes were blocked with 3% BSA (Beyotime, cat# ST023-200G) | Get A Quote |
Assessing impact on the hallmarks of aging has emerged as a novel method for prioritizing dual-purpose longevity therapeutic targets and developing drugs simultaneously targeting aging and disease. Cellular senescence, a central hallmark of aging, progressively induces cellular growth arrest and accelerates the production of a pro-inflammatory senescence-associated secretory phenotype (SASP). TGF- signaling is situated at the center of multiple senescence-associated and aging-associated signaling pathways, and its inhibition may be favorable for aging-related disorders. A recently developed Traf2- and Nck-interacting kinase (TNIK) inhibitor, INS018_055, was identified as a potent, novel anti-fibrotic agent affe... More
