| Products/Services Used | Details | Operation |
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| Peptide Synthesis | BioRad) were added to the remaining lysates and incubated for 2 h at 4 C. GST pulldown Pulldown with N-terminally biotinylated integrin 5 tail peptides (GenScript) was performed as follows: biotinylated peptides were incubated with streptavidin-conjugated Dynabeads (65001; Thermo Fisher Scientific) for 30 min at same way as described above for GFP-immunoprecipitated samples) from EGFP-GRASP65-overexpressing CHO cells or with recombinant GST-GRASP65 or GST alone (GenScript), at 4 C. Immunoblotting Immunoprecipitated complexes were washed three times with wash-buffer (20 mM Tris HCl pH 7.5, 150 mM NaCl, 1% NP-40) or RIPA | Get A Quote |
The study reveals that newly synthesized integrins can bypass the Golgi to be actively delivered to specific plasma membrane regions, where they enhance cell protrusion and adhesion. This targeted secretion rapidly increases integrin availability, giving cells dynamic flexibility to adjust to their environment.Constitutive integrin endocytosis and recycling control cell movement and morphology. In contrast, the role of newly synthesized integrins delivered via the biosynthetic pathway has been largely overlooked. We used the retention using selective hooks system to monitor the localization of new integrins exiting the endoplasmic reticulum in space and time. We discovered that new integrin delivery to the plas... More
